ADAM8's Role in Osteoclast Formation and Bone Resorption
Author Information
Author(s): Ishizuka Hisako, García-Palacios Verónica, Lu Ganwei, Subler Mark A, Zhang Heju, Boykin Christina S, Choi Sun Jin, Zhao Liena, Patrene Kenneth, Galson Deborah L, Blair Harry C, Hadi Tamer M, Windle Jolene J, Kurihara Noriyoshi, Roodman G David
Primary Institution: University of Pittsburgh Medical Center
Hypothesis
The study investigates the effects of ADAM8 on osteoclast formation and bone resorption in vivo and in vitro.
Conclusion
ADAM8 enhances osteoclast formation and bone resorption, suggesting it could be a therapeutic target for inflammatory bone diseases.
Supporting Evidence
- TRAP-ADAM8 mice showed increased osteoclast precursor numbers and enhanced bone-resorbing capacity.
- ADAM8 knockout mice did not display a bone phenotype but had blunted osteoclast formation in response to TNF-α.
- Enhanced expression of ADAM8 resulted in increased activation of signaling pathways involved in osteoclastogenesis.
Takeaway
ADAM8 helps bone cells called osteoclasts work better, which can lead to more bone being broken down, especially in diseases like arthritis.
Methodology
The study used transgenic and knockout mice to assess the role of ADAM8 in osteoclast formation and function through various assays.
Potential Biases
Potential bias in interpreting results from animal models may not translate directly to human physiology.
Limitations
The study primarily focuses on mouse models, which may not fully replicate human conditions.
Participant Demographics
Mice of various genotypes were used, including TRAP-ADAM8 transgenic and ADAM8 knockout mice.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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