Semen Collection and Cryopreservation in Griffon Vultures
Author Information
Author(s): Madeddu Manuela, Berlinguer Fiammetta, Ledda Massimo, Leoni Giovanni G, Satta Valentina, Succu Sara, Rotta Andrea, Pasciu Valeria, Zinellu Angelo, Muzzeddu Marco, Carru Ciriaco, Naitana Salvatore
Primary Institution: University of Sassari
Hypothesis
This study aimed to test the feasibility of a programme of semen collection and cryopreservation in Griffon vultures.
Conclusion
Semen cryopreservation can be considered a useful tool in the conservation of Griffon vulture genetic resources, but further studies are needed to optimize this technique.
Supporting Evidence
- Semen collection efficiency was low at 27.9%.
- Viability of thawed sperm was significantly lower than fresh semen.
- ATP concentration in frozen/thawed semen was significantly lower than in fresh semen.
Takeaway
Scientists collected and froze sperm from Griffon vultures to help save the species, but it was hard to get enough good sperm.
Methodology
Semen was collected using abdominal massage and evaluated for volume, concentration, viability, and ATP levels before and after freezing.
Potential Biases
Potential bias due to the small number of individuals and the stress of handling during semen collection.
Limitations
The study was limited by the small sample size and the challenges of collecting semen from wild-caught vultures.
Participant Demographics
Four male Griffon vultures aged between 3 and 30 years, kept in captivity due to unrecoverable traumas.
Statistical Information
P-Value
p < 0.001
Statistical Significance
p < 0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website