Transfer of MicroRNAs by Embryonic Stem Cell Microvesicles
Author Information
Author(s): Yuan Alex, Farber Erica L., Rapoport Ana Lia, Tejada Desiree, Deniskin Roman, Akhmedov Novrouz B., Farber Debora B.
Primary Institution: Jules Stein Eye Institute, UCLA School of Medicine, Los Angeles, California, United States of America
Hypothesis
Can embryonic stem cell microvesicles transfer microRNAs and proteins to other cells?
Conclusion
Embryonic stem cell microvesicles can transfer microRNAs and proteins to neighboring cells, potentially influencing gene expression.
Supporting Evidence
- Microvesicles can transfer proteins and RNA from cell to cell.
- Engineered microvesicles can carry exogenously expressed mRNA and protein.
- Microvesicles contain abundant microRNA and can transfer them to other cells.
Takeaway
Tiny bubbles from stem cells can carry important messages to other cells, helping them communicate and work together.
Methodology
The study isolated microvesicles from embryonic stem cells and analyzed their RNA and protein content, as well as their ability to transfer these materials to other cells.
Limitations
The study primarily focused on in vitro conditions, which may not fully represent in vivo environments.
Statistical Information
P-Value
p<0.0001
Confidence Interval
95th percentile confidence interval for each mRNA
Statistical Significance
p<0.0001
Digital Object Identifier (DOI)
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