Widespread Expression of BORIS/CTCFL in Normal and Cancer Cells
Author Information
Author(s): Tania A. Jones, Babatunji W. Ogunkolade, Jaroslaw Szary, Johan Aarum, Muhammad A. Mumin, Shyam Patel, Christopher A. Pieri, Denise Sheer
Primary Institution: Queen Mary University of London, Centre for Neuroscience and Trauma, Blizard Institute, Barts and the London School of Medicine and Dentistry, London, United Kingdom
Hypothesis
The expression pattern of BORIS is a significant and unresolved question in the field of DNA binding proteins.
Conclusion
BORIS expression is more widespread than previously believed and suggests a role for BORIS in nucleolar function.
Supporting Evidence
- BORIS is identified in the cytoplasm and nucleus of a wide range of normal and cancer cells.
- BORIS transcript levels are low compared to CTCF, but its protein levels are readily detectable.
- Live imaging confirmed the nucleolar accumulation of BORIS in transfected cells.
- Immunofluorescence staining showed enrichment of BORIS in the nucleolus of various cell types.
- Western blotting revealed similar levels of BORIS expression in normal and tumor cell lines.
Takeaway
BORIS is a protein that is found in many normal and cancer cells, especially in a part of the cell called the nucleolus, which helps make ribosomes.
Methodology
Real-time RT-PCR and Western blotting were used to analyze BORIS expression in various human tissues and cancer cell lines.
Limitations
The sensitivity of the assay may limit the detection of BORIS in certain tissues.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website