15-deoxy-Δ12,14-prostaglandin J2 activates TRPA1 and induces pain
Author Information
Author(s): Cruz-Orengo Lillian, Dhaka Ajay, Heuermann Robert J, Young Timothy J, Montana Michael C, Cavanaugh Eric J, Kim Donghee, Story Gina M
Primary Institution: Washington University Pain Center, Department of Anesthesiology, Washington University School of Medicine
Hypothesis
Does 15d-PGJ2 play a role in acute peripheral nociceptive signaling via TRPA1 activation?
Conclusion
15d-PGJ2 induces acute nociception when administered cutaneously and does so via a TRPA1-specific mechanism.
Supporting Evidence
- 15d-PGJ2 was identified as a TRPA1 agonist through a bioactive lipid library screen.
- Electrophysiological recordings confirmed that 15d-PGJ2 specifically activates TRPA1.
- Mice lacking TRPA1 did not exhibit nociceptive responses to 15d-PGJ2.
Takeaway
A substance called 15d-PGJ2 can make mice feel pain when it's put on their skin, and it does this by activating a special channel in their nerve cells.
Methodology
The study involved bioactive lipid library screening, electrophysiological recordings, and behavioral assays in mice.
Participant Demographics
Male C57BL6/J and TRPA1 mutant strain mice, aged 10-12 weeks.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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