New Test for Detecting Fasciola gigantica Infection
Author Information
Author(s): Demerdash Zeinab A, Diab Tarek M, Aly Ibrahim R, Mohamed Salwa H, Mahmoud Faten S, Zoheiry Mona K, Mansour Wafaa A, Attia Mohy E, El-Bassiouny Azza E
Primary Institution: Theodor Bilharz Research Institute, Giza, Egypt
Hypothesis
Can a monoclonal antibody-based ELISA effectively diagnose Fasciola gigantica infections in serum and stool samples?
Conclusion
The MoAb-based sandwich ELISA is more effective in detecting Fasciola gigantica in stool than in serum, providing a reliable, non-invasive diagnostic method.
Supporting Evidence
- The sensitivity of the ELISA in stool was 96%, while in serum it was 94%.
- The specificity of the assay was 98.2% in stool and 94.6% in serum.
- A positive correlation was found between stool ova count and ELISA OD readings.
Takeaway
This study created a new test that can find a specific parasite in poop better than in blood, making it easier to diagnose infections.
Methodology
The study used a monoclonal antibody-based sandwich ELISA to detect Fasciola gigantica antigens in serum and stool samples from infected patients.
Potential Biases
Potential bias in sample selection and the specificity of the antibodies used.
Limitations
The study may not account for variations in antigen detection due to different host sources or preparation methods.
Participant Demographics
Patients with abdominal pain and confirmed Fasciola gigantica infection, along with control groups of healthy individuals and those with other parasitic infections.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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