Treatment of EAE Reduces CD226 and T-bet+ CD4 T Cells in Periphery and Prevents Infiltration of T-bet+ IL-17, IFN-γ Producing T Cells into CNS
2011

RTL551 Treatment Reduces Pathogenic T Cells in EAE

Sample size: 8 publication 10 minutes Evidence: high

Author Information

Author(s): Sinha Sushmita, Miller Lisa M., Subramanian Sandhya, Burrows Gregory G., Vandenbark Arthur A., Offner Halina

Primary Institution: Oregon Health & Science University

Hypothesis

Does RTL551 treatment affect the maturation and infiltration of pathogenic T cells in experimental autoimmune encephalomyelitis (EAE)?

Conclusion

RTL551 treatment significantly reduces pathogenic T cells in the periphery and prevents their infiltration into the central nervous system during EAE.

Supporting Evidence

  • RTL551 treatment significantly reduced clinical signs of EAE.
  • RTL551 prevented the trafficking of pathogenic T cells from the spleen to the CNS.
  • RTL551 treatment resulted in lower frequencies of CD226 and T-bet expressing CD4+ T-cells in the blood.

Takeaway

This study shows that a treatment called RTL551 helps stop bad immune cells from causing damage in a disease called EAE, which is similar to multiple sclerosis.

Methodology

C57BL/6 mice were treated with RTL551 or control treatments after EAE induction, and various immune cell populations were analyzed using flow cytometry.

Potential Biases

Potential conflicts of interest due to financial ties of authors to a related company.

Limitations

The study is limited to a mouse model and may not fully translate to human conditions.

Participant Demographics

C57BL/6 male mice, 7-8 weeks old.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0021868

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