BEAP: The BLAST Extension and Alignment Program- a tool for contig construction and analysis of preliminary genome sequence
2009

BEAP: A Tool for Genome Sequence Analysis

publication Evidence: moderate

Author Information

Author(s): Koltes James E, Hu Zhi-Liang, Fritz Eric, Reecy James M

Primary Institution: Iowa State University

Hypothesis

Can BEAP effectively construct contigs for genomic regions in species with incomplete sequences?

Conclusion

The BEAP software successfully creates contigs for positional cloning and allows for graphical evaluation of sequence quality.

Supporting Evidence

  • BEAP allows for easy identification of sequence mismatches that may indicate SNPs.
  • The software can utilize both local and network databases for sequence assembly.
  • BEAP was tested on various template sequences to assess its performance.

Takeaway

BEAP is a computer program that helps scientists piece together bits of DNA to understand genes better, especially when they don't have the complete DNA sequence.

Methodology

BEAP combines BLAST and CAP3 to retrieve sequences and construct contigs for genomic regions.

Limitations

The software's performance may vary based on the E-value stringency and the quality of the input sequences.

Digital Object Identifier (DOI)

10.1186/1756-0500-2-11

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