Understanding How Dengue Virus Enters and Moves Inside Cells
Author Information
Author(s): Shrivastava Nidhi, Sripada Samatha, Kaur Jasmine, Shah Paresh S., Cecilia D.
Primary Institution: National Institute of Virology, Pune, India
Hypothesis
This study aims to understand the role of dynamin II, endosomes, microtubules, and dynein in the early events of DENV replication.
Conclusion
The study shows for the first time the association of dynamin II with DENV-2 during entry and dynein dependent retrograde trafficking of DENV proteins on microtubules.
Supporting Evidence
- Dynamin II was found to associate with the E protein of DENV-2 from 0 to 30 minutes post-infection.
- The association of E protein with dynein was confirmed by immunoprecipitation.
- Overexpression of dynamitin disrupted the trafficking of viral proteins.
- Viral RNA levels increased significantly from 12 hours post-infection.
- DENV-2 traffics within endosomes and is delivered to the perinuclear region within 30 minutes.
Takeaway
Dengue virus uses a helper protein to get inside cells and then rides along tiny tracks inside the cell to reach where it can make more viruses.
Methodology
The study used confocal microscopy to visualize internalization, endocytosis, and early trafficking of DENV-2 proteins in BHK-21 cells.
Digital Object Identifier (DOI)
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