Study of Immune Cells in Rag1 Mutant Zebrafish
Author Information
Author(s): Petrie-Hanson Lora, Hohn Claudia, Hanson Larry
Primary Institution: Mississippi State University
Hypothesis
What are the differences in leukocyte populations between rag1 mutant and wild-type zebrafish?
Conclusion
Rag1 mutant zebrafish have fewer lymphocyte-like cells and more granulocytes compared to wild-type zebrafish.
Supporting Evidence
- Rag1 mutants had significantly decreased lymphocyte-like cell populations compared to wild-types.
- Granulocyte populations were significantly increased in rag1 mutants compared to wild-types.
- RT-PCR showed rag1 mutants lacked expression of T cell receptor and immunoglobulin transcripts.
Takeaway
This study shows that mutant zebrafish have different types of immune cells than normal zebrafish, which helps us understand fish immunity better.
Methodology
The study involved differential leukocyte counts and flow cytometry analyses of zebrafish blood and kidney tissues.
Limitations
The study focused only on adult zebrafish and did not explore other developmental stages.
Participant Demographics
Adult zebrafish aged between 9 and 12 months.
Statistical Information
P-Value
p ≤ 0.05
Statistical Significance
p ≤ 0.05
Digital Object Identifier (DOI)
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