Markers of Primary Mesothelial Cells
Author Information
Author(s): Kanamori-Katayama Mutsumi, Kaiho Ai, Ishizu Yuri, Okamura-Oho Yuko, Hino Okio, Abe Masaaki, Kishimoto Takumi, Sekihara Hisahiko, Nakamura Yukio, Suzuki Harukazu, Forrest Alistair R. R., Hayashizaki Yoshihide
Primary Institution: OMICs Science Center, RIKEN Yokohama Institute, Yokohama, Kanagawa, Japan
Hypothesis
Can we identify specific biomarkers for primary mesothelial cells that could aid in the diagnosis of mesothelioma?
Conclusion
LRRN4 is a specific marker for primary mesothelial cells, while MSLN is more widely expressed in various cancer types.
Supporting Evidence
- LRRN4 was only detected in primary mesothelial cells.
- MSLN was detected in 15 of the 16 mesothelioma cell lines.
- UPK3B was detected in 6 of the mesothelioma cell lines.
Takeaway
The study found new markers that can help identify mesothelial cells, which are important for diagnosing a type of cancer called mesothelioma.
Methodology
Microarray expression arrays and qRT-PCR were used to identify and validate candidate mesothelial lineage markers.
Limitations
The study primarily focused on mouse models and may not fully represent human mesothelioma.
Participant Demographics
The study included human primary cells from two donors and various cancer cell lines.
Digital Object Identifier (DOI)
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