Regulation of the dpsA Gene in Streptomyces coelicolor
Author Information
Author(s): Facey Paul D., Sevcikova Beatrica, Novakova Renata, Hitchings Matthew D., Crack Jason C., Kormanec Jan, Dyson Paul J., Del Sol Ricardo
Primary Institution: Institute of Life Science, College of Medicine, Swansea University
Hypothesis
How is the expression of the dpsA gene regulated in response to environmental stress and during development in Streptomyces coelicolor?
Conclusion
The dpsA gene is regulated by multiple sigma factors, primarily SigB and SigH, in response to osmotic stress and during developmental differentiation.
Supporting Evidence
- The dpsA gene is transcribed from a single, sigB-like dependent promoter.
- Expression studies implicate SigH and SigB as the sigma factors responsible for dpsA expression.
- The promoter is massively induced in response to osmotic stress.
- WhiB is required for dpsA expression, particularly during development.
- Direct interaction between apoWhiB and the dpsA promoter region was demonstrated.
Takeaway
The dpsA gene helps Streptomyces coelicolor respond to stress and grow properly, and its activity is controlled by special proteins called sigma factors.
Methodology
The study used qRT-PCR, immunoblots, and gel retardation experiments to analyze dpsA expression and its regulation.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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