Adenovirus-mediated delivery of bFGF small interfering RNA reduces STAT3 phosphorylation and induces the depolarization of mitochondria and apoptosis in glioma cells U251
2011
Adenovirus-mediated delivery of bFGF siRNA reduces STAT3 activation and induces apoptosis in glioma cells
publication
Evidence: moderate
Author Information
Author(s): Liu Jun, Xu Xinnv, Feng Xuequan, Zhang Biao, Wang Jinhuan
Primary Institution: Tianjin Medical University
Hypothesis
The proliferation inhibition and apoptosis induction by Ad-bFGF-siRNA may result from the interruption of STAT3 phosphorylation.
Conclusion
Ad-bFGF-siRNA inhibits IL-6 secretion and reduces STAT3 phosphorylation, leading to apoptosis in glioma cells.
Supporting Evidence
- Ad-bFGF-siRNA reduces STAT3 phosphorylation at both Tyr705 and Ser727.
- IL-6 secretion is significantly lower in the Ad-bFGF-siRNA group compared to controls.
- Ad-bFGF-siRNA treatment leads to increased levels of apoptosis markers like Cytochrome C and Caspase3.
Takeaway
This study shows that a special virus can deliver a tiny piece of genetic material that helps stop cancer cells from growing and makes them die.
Methodology
The study involved infecting glioma cells with Ad-bFGF-siRNA and measuring various cellular responses, including STAT3 phosphorylation and apoptosis markers.
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.0001
Digital Object Identifier (DOI)
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