Suppression of Allograft Rejection by Tim-1-Fc through Cross-Linking with a Novel Tim-1 Binding Partner on T Cells
2011

Tim-1-Fc Inhibits Allograft Rejection

Sample size: 30 publication 10 minutes Evidence: high

Author Information

Author(s): Xiao Liang, Fu Zhi-ren, Liu Fang, Zhang Lu-ding, Shi Xiao-min, Shen Xiao-yun, Ni Zhi-jia, Fu Hong, Li Rui-dong, Cao Xue-tao, Ding Guo-shan, Wang Quan-xing

Primary Institution: Shanghai Changzheng Hospital, Second Military Medical University

Hypothesis

What is the role of Tim-1 in T-cell responses and allograft rejection?

Conclusion

Tim-1-Fc can inhibit T-cell responses and significantly prolong allograft survival.

Supporting Evidence

  • Tim-1-Fc selectively binds to CD4+ effector T cells.
  • Tim-1-Fc inhibits the proliferation of CD4+ T cells stimulated by allogeneic dendritic cells.
  • Treatment with Tim-1-Fc significantly prolonged cardiac allograft survival.
  • Tim-1-Fc treatment was associated with impaired Th1 response and preserved Th2 and nTregs function.
  • The frequency of Foxp3+ cells in splenic CD4+ T cells increased with Tim-1-Fc treatment.

Takeaway

Tim-1-Fc helps the body accept transplanted organs by stopping certain immune cells from attacking them.

Methodology

The study used recombinant human Tim-1 extracellular domain and IgG1-Fc fusion proteins to assess their effects on T-cell activation and allograft survival in mice.

Potential Biases

Potential bias in the interpretation of results due to the use of specific animal models.

Limitations

The study primarily focused on mouse models, which may not fully translate to human responses.

Participant Demographics

Male C57BL/6, BALB/c, and C3H mice aged 8–10 weeks.

Statistical Information

P-Value

p<0.01

Statistical Significance

p<0.01

Digital Object Identifier (DOI)

10.1371/journal.pone.0021697

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