Diolistics: incorporating fluorescent dyes into biological samples using a gene gun
2007
Diolistics: Using a Gene Gun to Incorporate Dyes into Cells
publication
Evidence: moderate
Author Information
Author(s): John A. O’Brien, Sarah C.R. Lummis
Primary Institution: MRC Laboratory of Molecular Biology, University of Cambridge
Hypothesis
Can the gene gun method improve the incorporation of fluorescent dyes into biological samples?
Conclusion
Diolistics offers a rapid and efficient way to label cells with fluorescent dyes, enhancing our ability to study cellular processes.
Supporting Evidence
- Diolistics allows for rapid labeling of cells with fluorescent dyes.
- The gene gun can transfect a wide range of tissues and organisms.
- Diolistics is non-invasive and can be used in living tissue.
Takeaway
Scientists can use a special tool called a gene gun to shoot tiny dyes into cells, helping them see and study the cells better.
Methodology
The gene gun propels DNA-coated microprojectiles into cells to deliver fluorescent dyes.
Limitations
The technique may cause some cell damage and requires optimization for different tissue types.
Digital Object Identifier (DOI)
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