Creating a Human Antibody to Target Yeast Cytosine Deaminase
Author Information
Author(s): Mallano Alessandra, Zamboni Silvia, Carpinelli Giulia, Santoro Filippo, Flego Michela, Ascione Alessandro, Gellini Mara, Tombesi Marina, Podo Franca, Cianfriglia Maurizio
Primary Institution: Istituto Superiore di SanitÃ
Hypothesis
Can a human monoclonal antibody in scFv format be developed to detect yeast cytosine deaminase without interfering with its enzymatic function?
Conclusion
The scFvH5 antibody can detect yeast cytosine deaminase in laboratory techniques without affecting its ability to convert 5-fluorocytosine into 5-fluorouracil.
Supporting Evidence
- The scFvH5 antibody was isolated from a large phage display library.
- The binding of scFvH5 to yeast cytosine deaminase was confirmed by Western blot and ELISA.
- The enzyme activity of yeast cytosine deaminase was not affected by the presence of scFvH5.
Takeaway
Scientists made a special human antibody that can find a yeast enzyme used in cancer treatment without stopping it from working.
Methodology
The study involved expressing a recombinant yeast cytosine deaminase in E. coli, isolating specific antibodies from a phage display library, and testing their binding and functional effects.
Digital Object Identifier (DOI)
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