Genome Structure of and Genome-Wide Recombination in the Saccharomyces cerevisiae S288C Progenitor Isolate EM93
2011

Genomic Structure and Recombination in Saccharomyces cerevisiae EM93

Sample size: 480 publication 10 minutes Evidence: high

Author Information

Author(s): Anders Esberg, Ludo A. H. Muller, John H. McCusker

Primary Institution: Department of Molecular Genetics and Microbiology, Duke University Medical Center

Hypothesis

How do genetic differences between the EM93 isolate and the S288C strain affect meiotic recombination?

Conclusion

The study reveals that EM93 has significant genetic differences from S288C, affecting recombination rates and patterns.

Supporting Evidence

  • EM93 is heterozygous for over 45,000 polymorphisms.
  • The average EM93 meiosis produces 144 detectable recombination events.
  • Non-telomeric heterozygous LSPs significantly reduce meiotic recombination in adjacent intervals.
  • 203 recombination hotspots were identified in the EM93 genome.

Takeaway

Scientists studied a type of yeast to see how its genes are different from another type, which helps understand how they mix genes when they reproduce.

Methodology

The researchers used Tiling Arrays and custom genotyping arrays to analyze genetic markers and recombination events in EM93.

Potential Biases

The study may be biased due to the specific strains used and the methods of detecting recombination.

Limitations

The genotyping array covered only 77% of the EM93 genome, potentially missing some recombination events.

Participant Demographics

The study focused on the yeast strain EM93 and its genetic characteristics.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0025211

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