Regulation of Gene Expression in Yeast Under Stress
Author Information
Author(s): Craig Lawless, Richard D Pearson, Julian N Selley, Julia B Smirnova, Christopher M Grant, Mark P Ashe, Graham D Pavitt, Simon J Hubbard
Primary Institution: University of Manchester
Hypothesis
The study investigates the role of upstream sequence elements in regulating gene expression under stress conditions in yeast.
Conclusion
The results demonstrate that UTR sequences, particularly 5' UTRs and uORFs, play a significant role in post-transcriptional control of gene expression in yeast under stress.
Supporting Evidence
- The study shows a significant enrichment in specific GO functional classes for genes that are translationally regulated under stress.
- Translationally upregulated genes have longer 5' UTRs, indicating their role in translational regulation.
- uORFs are statistically under-represented in UTR sequences but are more common in genes that are translationally up-regulated.
Takeaway
This study shows that parts of the gene that are not used to make proteins can help control how much protein is made, especially when yeast cells are stressed.
Methodology
The study used large-scale datasets to analyze the role of UTR sequences and uORFs in translational regulation under various stress conditions.
Limitations
uORFs are relatively rare, and many unanswered questions remain about how UTR elements direct translational control.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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