Molecular Characterization of Viruses from Clinical Respiratory Samples Producing Unidentified Cytopathic Effects in Cell Culture
2009

Identifying Viruses in Respiratory Samples Using SISPA

Sample size: 17 publication Evidence: moderate

Author Information

Author(s): Yacine Abed, Guy Boivin

Primary Institution: Research Center in Infectious Diseases, CHUQ-CHUL and Laval University

Hypothesis

Can the SISPA method effectively identify viruses producing uncharacterized cytopathic effects in clinical respiratory samples?

Conclusion

The SISPA method successfully identified six viruses in 17 clinical respiratory samples that exhibited uncharacterized cytopathic effects.

Supporting Evidence

  • The SISPA method identified six viruses from 17 clinical samples.
  • Five out of the six identified viruses belonged to the Picornaviridae family.
  • The identified viruses included influenza C, HPeV-3, and cardiovirus Saffold.

Takeaway

Researchers used a special method called SISPA to find viruses in sick people's samples, and they discovered six different viruses.

Methodology

The SISPA method was used to analyze 17 clinical respiratory samples for virus identification.

Limitations

Only 6 out of 17 cases were successfully identified, indicating limitations in the SISPA method's effectiveness.

Participant Demographics

Samples included respiratory specimens from patients of varying ages, including children and adults.

Digital Object Identifier (DOI)

10.3390/v1020084

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