Detachment of Early Stage Biofilm from Candida albicans
Author Information
Author(s): Sellam Adnane, Al-Niemi Thamir, McInnerney Kathleen, Brumfield Susan, Nantel Andre, Suci Peter A
Primary Institution: Biotechnology Research Institute, National Research Council of Canada
Hypothesis
Is C. albicans biofilm detachment from a biomaterial surface actively regulated at the level of transcription?
Conclusion
The detachment of the early stage biofilm from a biomaterial surface is not primarily controlled by transcriptional regulation.
Supporting Evidence
- Dispersal from C. albicans biofilms is linked to bloodstream infections.
- Biofilms showed a complex gene expression pattern during detachment.
- Detachment was not influenced by oxygen availability at the interface.
Takeaway
Candida albicans can stick to surfaces and form a biofilm, but as it grows, it can let go and spread, which is important for understanding infections.
Methodology
Biofilms were cultured in a flow-through system, and transcriptome responses were analyzed at various time points.
Limitations
The study did not identify a single gene responsible for the detachment process, indicating complexity in the regulation.
Statistical Information
P-Value
1.4e-6
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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