Characterizing the Host and Symbiont Proteomes in the Association between the Bobtail Squid, Euprymna scolopes, and the Bacterium, Vibrio fischeri
2011

Understanding the Proteomes of the Bobtail Squid and Its Bacterium

Sample size: 7 publication 10 minutes Evidence: high

Author Information

Author(s): Tyler R. Schleicher, Spencer V. Nyholm

Primary Institution: Department of Molecular and Cell Biology, University of Connecticut

Hypothesis

What are the contributions of the host and symbiont proteomes in the squid-Vibrio fischeri symbiosis?

Conclusion

The study identified 1581 unique proteins from both the squid and its symbiotic bacterium, revealing important roles in immune response and symbiosis regulation.

Supporting Evidence

  • The study identified 870 symbiont proteins and 711 host proteins.
  • Proteins related to the innate immune system were found in the host.
  • Symbiont proteins involved in quorum sensing and detoxification were identified.
  • The research provides the first proteomic analysis of the squid's light organ.

Takeaway

This study looked at the proteins in a squid and its bacteria to see how they help each other live together. They found a lot of important proteins that help keep the relationship healthy.

Methodology

Proteomic analyses using 1D- and 2D-polyacrylamide gel electrophoresis and multidimensional protein identification technology (MudPIT).

Digital Object Identifier (DOI)

10.1371/journal.pone.0025649

Want to read the original?

Access the complete publication on the publisher's website

View Original Publication