Prostaglandin E2-EP1 and EP2 receptor signaling promotes apical junctional complex disassembly of Caco-2 human colorectal cancer cells
2008
Prostaglandin E2 and Its Role in Disassembling Cell Junctions in Colorectal Cancer Cells
publication
Evidence: moderate
Author Information
Author(s): Tanaka Marcelo N, Diaz Bruno L, de Souza Wanderley, Morgado-Diaz Jose A
Primary Institution: Instituto Nacional de Câncer
Hypothesis
PGE2 would impair the AJC assembly and function of Caco-2 cells.
Conclusion
PGE2-EP1 and EP2 receptor signaling mediates AJC disassembly through a mechanism involving PKC and claudin-1.
Supporting Evidence
- PGE2 treatment caused a significant drop in transepithelial electrical resistance.
- Immunofluorescence showed altered distribution of AJC proteins after PGE2 treatment.
- PKC inhibition prevented the effects of PGE2 on AJC disassembly.
Takeaway
This study shows that a substance called PGE2 can change how cells stick together in the gut, which might help cancer grow.
Methodology
Caco-2 cells were treated with PGE2, and the effects on AJC protein distribution and barrier function were analyzed using immunofluorescence, immunoblotting, and electron microscopy.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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