A relationship between slide quality and image quality in whole slide imaging (WSI)
2008
Impact of Tissue Section Thickness on Whole Slide Imaging Quality
publication
Evidence: moderate
Author Information
Author(s): Yagi Yukako, Gilbertson John R
Primary Institution: Department of Pathology, Harvard Medical School
Hypothesis
Thinner, more consistent tissue sections improve whole slide imaging capture speed and image quality.
Conclusion
Thinner tissue sections lead to faster capture times and better image quality in whole slide imaging.
Supporting Evidence
- Thinner sections required significantly fewer focus points.
- Thinner sections had significantly lower capture times by 10–15%.
- Image quality was judged to be significantly better with thinner sections.
Takeaway
If you cut tissue samples thinner, the pictures taken by machines will be clearer and faster to make.
Methodology
Tissue types were sectioned at varying thicknesses and imaged with different WSI devices to compare image quality and scanning speed.
Limitations
The automated tissue-sectioning machine is not yet available in US or European markets.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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