Contribution of the Lipopolysaccharide to Resistance of Shigella flexneri 2a to Extreme Acidity
2011

How Shigella flexneri Resists Acidic Environments

publication Evidence: high

Author Information

Author(s): Martinić Mara, Hoare Anilei, Contreras Inés Álvarez, Sergio A.

Primary Institution: Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile

Hypothesis

The lipopolysaccharide (LPS) of Shigella flexneri plays a crucial role in its resistance to extreme acidity.

Conclusion

The study found that both the O antigen and modifications to lipid A are essential for Shigella flexneri's resistance to extreme acid conditions.

Supporting Evidence

  • A mutant lacking O antigen was significantly more sensitive to extreme acid conditions than the wild type.
  • Glucosylation of the O antigen contributed to acid resistance.
  • Modifications to lipid A increased resistance to extreme acid conditions.

Takeaway

Shigella flexneri can survive in very acidic environments because of special parts on its surface that help protect it.

Methodology

The researchers created mutant strains of Shigella flexneri and tested their survival in acidic conditions after adaptation to moderate acidity.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0025557

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