Producing Human Acetylcholinesterase in Hairy Root Cultures
Author Information
Author(s): Ryan R Woods, Brian C Geyer, Tsafrir S Mor
Primary Institution: Arizona State University
Hypothesis
Can hairy root organ cultures derived from transgenic plants effectively produce human acetylcholinesterase?
Conclusion
The study shows that plant organ cultures can successfully produce human acetylcholinesterase, offering a viable alternative to traditional production methods.
Supporting Evidence
- The hairy root cultures accumulated acetylcholinesterase to levels up to 3.3% of total soluble protein.
- The enzyme produced was biochemically and functionally similar to that derived from transgenic plants.
- Hairy root cultures can be stored at 4°C for up to 5 months.
Takeaway
Scientists found a way to grow a special plant root that makes a useful human protein, which can help protect against certain poisons.
Methodology
Hairy root cultures were created from transgenic Nicotiana benthamiana plants and screened for acetylcholinesterase production.
Limitations
The hairy root cultures had slower growth rates and lower maximum densities compared to wild type cultures.
Digital Object Identifier (DOI)
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