Enzyme production by filamentous fungi: analysis of the secretome of Trichoderma reesei grown on unconventional carbon source
2011
Enzyme Production by Trichoderma reesei on Different Growth Media
publication
Evidence: moderate
Author Information
Author(s): Jun He, Thomas Kieselbach, Leif Jönsson
Primary Institution: Umeå University
Hypothesis
Can spent hydrolysates serve as an effective growth medium for enzyme production in Trichoderma reesei?
Conclusion
The study found that Trichoderma reesei can effectively produce cellulolytic enzymes using spent hydrolysates as a growth medium.
Supporting Evidence
- Both spent hydrolysate and lactose-based media supported good growth of Trichoderma reesei.
- 52 protein spots were identified, indicating a diverse secretome.
- Cellobiohydrolases were the most abundant enzymes produced in both media.
Takeaway
This study shows that a type of fungus can grow well and make useful enzymes when fed with leftover plant materials.
Methodology
Proteomics was used to analyze the secretome of Trichoderma reesei grown on spent hydrolysate and lactose-based media.
Limitations
The enzyme mixtures produced in spent hydrolysates are not well characterized, and the study did not explore all potential carbon sources.
Digital Object Identifier (DOI)
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