Cyclic Changes of C/EBP Beta in Human Endometrium
Author Information
Author(s): Beth J Plante, Kannan Athilakshmi, Milan K Bagchi, Lingwen Yuan, Steven L Young
Primary Institution: University of North Carolina School of Medicine
Hypothesis
To characterize changes in C/EBP beta mRNA abundance and protein localization over the normal human menstrual cycle.
Conclusion
C/EBP beta mRNA and protein expression change throughout the normal human menstrual cycle, particularly during the embryo-receptive phase.
Supporting Evidence
- C/EBP beta mRNA expression peaks in the late secretory phase.
- Nuclear C/EBP beta protein increases in stromal cells around cycle day 20.
- Significant changes in nuclear C/EBP beta expression correlate with endometrial receptivity.
Takeaway
This study looked at how a protein called C/EBP beta changes in the uterus during a woman's monthly cycle, which might help with understanding how the body gets ready for pregnancy.
Methodology
Endometrial samples were taken from 50 volunteers at different cycle phases and analyzed for C/EBP beta mRNA expression using RT-PCR and protein localization using immunohistochemistry.
Potential Biases
Potential bias in sample selection and observer interpretation of histological scores.
Limitations
The study may not account for individual variations in hormone levels and cycle phases.
Participant Demographics
50 normal volunteers aged 18-35 with regular menstrual cycles.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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