How Hoxd4 and miR-10b Work Together in Mouse Embryo Development
Author Information
Author(s): Phua Sze Lynn Calista, Sivakamasundari V., Shao Yu, Cai Xiaohan, Zhang Li-Feng, Lufkin Thomas, Featherstone Mark
Primary Institution: School of Biological Sciences, Nanyang Technological University, Singapore
Hypothesis
The expression of Hoxd4 and miR-10b is co-ordinately regulated during neural differentiation.
Conclusion
Hoxd4 and miR-10b transcripts are generated by Drosha cleavage and are co-regulated during neural differentiation.
Supporting Evidence
- The expression profiles of Hoxd4 and miR-10b transcripts during neural differentiation of mouse embryonal carcinoma cells are co-ordinately regulated.
- P1 transcripts are generated by Drosha cleavage of P2 transcripts, indicating a novel regulatory mechanism.
- Uncapped P1 transcripts accumulate in the nucleus, suggesting a potential functional role.
Takeaway
This study shows that two important genes, Hoxd4 and miR-10b, work together in developing mouse embryos, helping to control how cells become different types of tissues.
Methodology
The study used quantitative reverse transcriptase PCR (qRT-PCR) and RNA-ligase mediated 5′ Rapid Amplification of cDNA Ends (5′ RLM-RACE) to analyze transcript levels and origins.
Limitations
The study does not explore the functional implications of the uncapped transcripts in detail.
Digital Object Identifier (DOI)
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