Sorting of Early and Late Flagellar Subunits After Docking at the Membrane ATPase of the Type III Export Pathway
2007
Sorting of Flagellar Subunits in Bacteria
publication
Evidence: moderate
Author Information
Author(s): Stafford Graham P., Evans Lewis D.B., Krumscheid Rita, Dhillon Paraminder, Fraser Gillian M., Hughes Colin
Primary Institution: Cambridge University Department of Pathology
Hypothesis
Are subunit docking and sorting separable events in the type III export pathway?
Conclusion
The study shows that early and late flagellar subunits converge at the FliI ATPase before being sorted for export.
Supporting Evidence
- The study indicates that both early and late subunits can accumulate at the membrane ATPase.
- Chaperoned late subunits engage the FliI ATPase before sorting.
- The N-terminal regions of subunits contain sufficient information to determine sorting.
Takeaway
Bacteria have a special way to build their flagella, and this study found that the parts are sorted at a specific point before they are sent out.
Methodology
The export pathway was manipulated in vivo to observe the sorting of early and late flagellar subunits.
Digital Object Identifier (DOI)
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