Valproic Acid Enhances Ventral Patterning of Human Neural Tube Organoids
Author Information
Author(s): Zheng Yuanyuan, Zhang Fangrong, Nie Haifeng, Li Xinyu, Xun Jiali, Fu Jianping, Wu Lijun
Primary Institution: Institute of Physical Science and Information Technology Anhui University
Hypothesis
Modulation of valproic acid in the human neural tube organoids culture will promote dorsal differentiation of hNTOs.
Conclusion
Valproic acid enhances the generation of more reproducible, ventrally self-patterned human neural tube organoids by inducing Wnt signaling and suppressing Shh signaling.
Supporting Evidence
- VPA treatment increased the efficiency of ventrally patterned hNTOs from 15% to 41%.
- RNA sequencing revealed significant upregulation of Wnt signaling genes in VPA-treated organoids.
- Western-blot analysis confirmed increased levels of GLI3R proteins after VPA treatment.
Takeaway
Valproic acid helps make better brain organoids by changing how certain signals work, which helps them develop properly.
Methodology
The study used RNA sequencing and RT-qPCR to analyze gene expression changes in human neural tube organoids treated with valproic acid.
Limitations
The study did not compare the effects of Wnt signaling specific activators and valproic acid on dorsal and ventral patterning of hNTOs.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website