How a Drug Affects Cold Sensation in Mice
Author Information
Author(s): Wendy M. Knowlton, Richard L. Daniels, Radhika Palkar, Daniel D. McCoy, David D. McKemy
Primary Institution: University of Southern California
Hypothesis
The study investigates the effects of a TRPM8 antagonist on cold sensation and thermoregulation in mice.
Conclusion
The TRPM8 antagonist PBMC significantly alters cold sensation and thermoregulation in mice.
Supporting Evidence
- PBMC selectively blocks TRPM8 activity in vitro and in vivo.
- PBMC administration resulted in dose-dependent hypothermia in wildtype mice.
- TRPM8-knockout mice showed no response to PBMC treatment.
Takeaway
Researchers tested a drug that changes how mice feel cold, and it worked really well, making them less sensitive to cold temperatures.
Methodology
The study used calcium microfluorimetry and whole-cell voltage clamp recordings to assess the effects of PBMC on TRPM8 channels in vitro and in vivo.
Limitations
The study did not test higher doses of PBMC due to significant effects on thermoregulation.
Participant Demographics
Adult mice aged at least eight weeks, including wildtype and TRPM8-knockout mice.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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