DNA Damage from Boron Neutron Capture Reaction in Ku80-Deficient Cells
Author Information
Author(s): Kinashi Yuko, Takahashi Sentaro, Kashino Genro, Okayasu Ryuichi, Masunaga Shinichiro, Suzuki Minoru, Ono Koji
Primary Institution: Research Reactor Institute, Kyoto University
Hypothesis
Boron neutron capture reaction (BNCR) will increase cell killing and slow disappearance of repair protein-related foci to a greater extent in DNA repair-deficient cells than in wild-type cells.
Conclusion
BNCR induces high cytotoxic effects and low capacity to repair DNA double-strand breaks in Ku80-deficient cells.
Supporting Evidence
- The number of gamma-H2AX foci in xrs-5 cells at 60-120 min after BNCT correlated with the cell killing effect of BNCR.
- In contrast, foci levels in the xrs-5 cells were significantly higher than in CHO-K1 cells two hours after irradiation.
- The RBE following BNCR of radio-sensitive mutant cells was lower than that of radio-resistant cells.
Takeaway
This study shows that certain cells that can't repair their DNA are more affected by a special type of radiation treatment, making them more sensitive to it.
Methodology
CHO-K1 and xrs-5 cells were irradiated by thermal neutrons, and DNA double-strand breaks were evaluated by measuring gamma-H2AX and 53BP1 foci using immunofluorescence.
Limitations
The study primarily focuses on two cell lines, which may limit the generalizability of the findings.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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