Evaluation and optimization of membrane feeding compared to direct feeding as an assay for infectivity
2008

Comparing Feeding Methods for Malaria Transmission Studies

Sample size: 372 publication Evidence: moderate

Author Information

Author(s): Diallo Mouctar, Touré Abdoulaye M, Traoré Sekou F, Niaré Oumou, Kassambara Lalla, Konaré Awa, Coulibaly Mamadou, Bagayogo Magaran, Beier John C, Sakai Richard K, Touré Yéya T, Doumbo Ogobara K

Primary Institution: Malaria Research and Training Center (MRTC), University of Bamako, Bamako, Mali

Hypothesis

Is membrane feeding as effective as direct feeding for assessing malaria infectivity in mosquitoes?

Conclusion

Membrane feeding is less effective than direct feeding for infecting mosquitoes, but could be a viable alternative for evaluating malaria vaccine transmission-blocking activity.

Supporting Evidence

  • Children aged 4–9 years were found to be more infectious than adolescents.
  • Direct feeding resulted in a significantly higher percentage of mosquitoes becoming infected compared to membrane feeding.
  • Infectivity was about three times higher for direct feeding than for membrane feeding.

Takeaway

This study looked at two ways to feed mosquitoes to see which one makes them more likely to get malaria from people. It found that feeding them directly is much better than using a special membrane.

Methodology

The study compared direct and membrane feeding methods for infectivity using gametocyte carriers in a rural area of Mali.

Potential Biases

Potential bias due to the ethical concerns of exposing children to malaria through direct feeding.

Limitations

Data from 1996 were not included in the analysis, and the study was limited to a specific geographic area.

Participant Demographics

Children and adolescents aged 4 to 18 years from Bancoumana, Mali.

Statistical Information

P-Value

0.039

Statistical Significance

p < 0.001

Digital Object Identifier (DOI)

10.1186/1475-2875-7-248

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