Structural and functional roles of deamidation of N146 and/or truncation of NH2- or COOH-termini in human αB-crystallin
2011
Effects of Deamidation and Truncation on Human αB-Crystallin
publication
Evidence: moderate
Author Information
Author(s): Asomugha C.O., Gupta R., Srivastava O.P.
Primary Institution: University of Alabama at Birmingham
Hypothesis
What are the relative effects of deamidation and/or truncation on the structural and functional properties of αB-crystallin?
Conclusion
Deamidation and truncation significantly alter the structural properties and chaperone activity of αB-crystallin.
Supporting Evidence
- All αB mutants showed higher molecular mass compared to WT αB.
- Chaperone activity decreased significantly in deamidated and truncated mutants.
- Structural changes were confirmed by CD spectroscopy and fluorescence studies.
Takeaway
This study looks at how changes to a protein called αB-crystallin affect its ability to help other proteins stay healthy, which is important for eye health.
Methodology
The study involved generating various mutants of αB-crystallin and comparing their structural and functional properties using techniques like CD spectroscopy and fluorescence studies.
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