Early Detection of Schistosomiasis in Mice Using Antibody Responses
Author Information
Author(s): Wang Jie, Yu Chuan-Xin, Yin Xu-Ren, Zhang Wei, Qian Chun-Yan, Song Li-Jun, Ke Xue-Dan, Xu Yong-Liang, He Wei, Cao Guo-Qun
Primary Institution: Jiangsu Institute of Parasitic Diseases, Wuxi, People's Republic of China
Hypothesis
Can antibody responses to the Sj23HD protein and SEA be used for early detection of schistosomiasis in sentinel mice?
Conclusion
Antibody responses to the Sj23HD antigen can be effectively monitored for early detection of schistosome infection in mice.
Supporting Evidence
- Antibody levels against Sj23HD appeared earlier than those against SEA.
- Immunoblotting showed greater sensitivity and specificity than ELISA for detecting Sj23HD antibodies.
- Positive antibody responses correlated with the load of cercariae and duration of infection.
Takeaway
Scientists found that mice infected with a parasite showed antibodies earlier when tested for a specific protein, helping to catch infections sooner.
Methodology
Mice were infected with cercariae and antibody levels were measured over 42 days using ELISA and immunoblotting.
Potential Biases
Potential for cross-reactivity with non-specific proteins in the antigens used.
Limitations
The study primarily focused on a specific protein and may not account for all variations in immune response.
Participant Demographics
ICR mice, female, 20 g, 6 weeks old.
Digital Object Identifier (DOI)
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