Peripheral CLOCK Regulates Target-Tissue Glucocorticoid Receptor Transcriptional Activity in a Circadian Fashion in Man
2011

How the Body's Clock Affects Stress Hormones

Sample size: 10 publication 10 minutes Evidence: moderate

Author Information

Author(s): Charmandari Evangelia, Chrousos George P., Lambrou George I., Pavlaki Aikaterini, Koide Hisashi, Ng Sinnie Sin Man, Kino Tomoshige

Primary Institution: First Department of Pediatrics, University of Athens Medical School, “Aghia Sophia” Children's Hospital, Athens, Greece

Hypothesis

The study investigates how the peripheral CLOCK system regulates glucocorticoid receptor activity in a circadian manner in humans.

Conclusion

The peripheral CLOCK system may decrease tissue sensitivity to glucocorticoids in the morning and increase it at night.

Supporting Evidence

  • GR acetylation was higher in the morning than in the evening.
  • Some glucocorticoid-responsive genes did not show expected diurnal fluctuations in vivo.
  • Hydrocortisone treatment affected mRNA expression of CLOCK-related genes in non-synchronized cells.

Takeaway

Our body's internal clock helps control how sensitive we are to stress hormones like cortisol, making us less sensitive in the morning and more sensitive at night.

Methodology

The study examined GR acetylation and mRNA expression in peripheral blood mononuclear cells from healthy subjects at different times of the day.

Limitations

The study only included healthy subjects, which may limit the generalizability of the findings to other populations.

Participant Demographics

10 healthy subjects (3 males, 7 females, mean age 33.3 years)

Statistical Information

P-Value

p<0.01

Statistical Significance

p<0.01

Digital Object Identifier (DOI)

10.1371/journal.pone.0025612

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