Engineering Therapeutic Proteins in E. coli
Author Information
Author(s): Kamionka Mariusz
Primary Institution: Trinity College, University of Dublin
Hypothesis
Can protein engineering in E. coli overcome the limitations of posttranslational modifications for therapeutic proteins?
Conclusion
Protein engineering can enhance the production and stability of therapeutic proteins in E. coli despite the lack of certain posttranslational modifications.
Supporting Evidence
- E. coli is a low-cost and simple system for producing therapeutic proteins.
- Protein engineering can improve the stability and biological activity of proteins.
- Genetic fusions can enhance the solubility and half-life of therapeutic proteins.
Takeaway
Scientists can change proteins made in bacteria to make them work better, even though bacteria can't do some of the fancy changes that our bodies do.
Methodology
The article reviews various strategies for engineering therapeutic proteins in E. coli, including genetic modifications and protein fusions.
Limitations
The lack of posttranslational modifications in E. coli can limit the biological activity of some proteins.
Digital Object Identifier (DOI)
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