Still stratus not altocumulus: Further evidence against the date/party hub distinction
2007

Evidence Against the Date/Party Hub Distinction in Yeast Protein Interaction Networks

Sample size: 181 publication Evidence: high

Author Information

Author(s): Nizar N. Batada, Teresa Reguly, Ashton Breitkreutz, Lorrie Boucher, Bobby-Joe Breitkreutz, Mike Tyers, Laureance D. Hurst

Primary Institution: University of Bath

Hypothesis

Is the yeast protein interaction network made up of two distinct types of hubs, party and date?

Conclusion

The study found no evidence supporting the distinction between party and date hubs in yeast protein interactions.

Supporting Evidence

  • The study analyzed 25 expression datasets across seven protein interaction builds.
  • Statistical tests showed no evidence for bimodality in co-expression values.
  • Deletion of nonessential date and party hubs had identical effects on network integrity.
  • Genetic connectivity differences between party and date hubs diminished when controlling for study bias.

Takeaway

The researchers looked at how proteins interact in yeast and found that the idea of two types of hubs, like party and date, doesn't hold up.

Methodology

The study analyzed multiple high-confidence datasets of protein interactions using statistical tests to evaluate the presence of bimodality and other characteristics.

Potential Biases

Potential study bias was noted in the analysis of genetic interactions.

Limitations

The original dataset was small, and some analyses were based on visual inspection rather than formal statistical tests.

Participant Demographics

The study focused on yeast proteins, specifically Saccharomyces cerevisiae.

Statistical Information

P-Value

0.00006

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pbio.0050154

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