Prohibitin Overexpression in Hepatitis C Virus-Infected Cells
Author Information
Author(s): Dang Shuang-Suo, Sun Ming-Zhu, Yang E, Xun Meng, Ma Li, Jia Zhan-Sheng, Wang Wen-Jun, Jia Xiao-Li
Primary Institution: Xi'an Jiao Tong University
Hypothesis
The up-regulated proteins by HCV infection have a direct connection with the pathological liver injury.
Conclusion
Prohibitin expression is significantly higher in Huh-7-HCV and Huh-7.5-HCV cells compared to their control counterparts.
Supporting Evidence
- Prohibitin mRNA levels were 2.09 times higher in Huh-7-HCV cells compared to Huh-7 cells.
- Prohibitin protein levels were 2.38 times higher in Huh-7-HCV cells compared to Huh-7 cells.
- Prohibitin mRNA levels were 2.25 times higher in Huh-7.5-HCV cells compared to Huh-7.5 cells.
- Prohibitin protein levels were 2.29 times higher in Huh-7.5-HCV cells compared to Huh-7.5 cells.
Takeaway
This study found that a protein called prohibitin is much more present in liver cells infected with the hepatitis C virus, which might help us understand how the virus causes liver damage.
Methodology
The study used real-time PCR and western blotting to assess prohibitin expression at both mRNA and protein levels in Huh-7 and Huh-7.5 cells infected with HCV.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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