Conservation in the face of diversity: multistrain analysis of an intracellular bacterium
2009

Genomic Diversity in Anaplasma marginale

Sample size: 5 publication 10 minutes Evidence: moderate

Author Information

Author(s): Michael J Dark, David R Herndon, Lowell S Kappmeyer, Mikel P Gonzales, Elizabeth Nordeen, Guy H Palmer, Donald P Knowles Jr, Kelly A Brayton

Primary Institution: Washington State University

Hypothesis

What is the level of genomic diversity among different strains of Anaplasma marginale?

Conclusion

The study found that Anaplasma marginale has a closed-core genome with variable SNP retention rates among strains.

Supporting Evidence

  • A. marginale has a closed-core genome with few highly plastic regions.
  • SNPs comprise 0.8% of the longer Florida genome.
  • The study compared genomes from multiple strains to assess diversity.

Takeaway

Scientists looked at different strains of a bacteria called Anaplasma marginale to see how much they differ from each other. They found that while some parts of the bacteria's DNA are very similar, there are also many small changes that make each strain unique.

Methodology

The complete genome sequence of the Florida strain of Anaplasma marginale was determined, along with near-complete sequences for three additional strains, for comparative analysis.

Potential Biases

Selection of strains based on specific phenotypic traits may skew the perceived level of genetic diversity.

Limitations

The study's findings may not represent the true diversity of Anaplasma marginale due to selection bias in strain selection.

Digital Object Identifier (DOI)

10.1186/1471-2164-10-16

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