Impact of GW/P Body Components on Primary Ciliogenesis in Human Astrocytes
Author Information
Author(s): Joanna J. Moser, Marvin J. Fritzler, Jerome B. Rattner
Primary Institution: University of Calgary
Hypothesis
Does the inhibition of the miRNA pathway impair primary cilium formation in human astrocytes?
Conclusion
The study demonstrates that repression of key proteins in the miRNA pathway impairs the formation of primary cilia in human astrocytes.
Supporting Evidence
- GW/P bodies localize to the centrosome in human astrocytes.
- Knockdown of GW182 and hAgo2 resulted in the absence of primary cilia.
- Ultrastructural analysis confirmed that early stages of ciliogenesis were not affected by the knockdown.
Takeaway
This study found that certain proteins help cells grow tiny hair-like structures called cilia, and when these proteins are blocked, the cilia can't form properly.
Methodology
Human astrocytes were transfected with siRNA targeting GW182 and hAgo2, and primary cilia were examined using indirect immunofluorescence.
Limitations
Current techniques do not allow for specific targeting of the two GW/P bodies at the centrosome.
Participant Demographics
Primary human astrocytes and synoviocytes were used in the study.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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