Changes in Kir4.1 and AQP4 Expression in Rat Retina During Inflammation
Author Information
Author(s): Liu Xiao-Qiang, Kobayashi Hideyuki, Jin Zi-Bing, Wada Akihiko, Nao-i Nobuhisa
Primary Institution: University of Miyazaki
Hypothesis
The study investigates the expression of Kir4.1 and AQP4 in the retina during endotoxin-induced uveitis in rats.
Conclusion
The study found that endotoxin-induced uveitis alters the expression of Kir4.1 and AQP4 differently, which may contribute to retinal edema during inflammation.
Supporting Evidence
- The expression of Kir4.1 mRNA decreased sharply, reaching a minimum at 12 hours after LPS injection.
- The expression of Kir4.1 protein decreased significantly, reaching a minimum at three days after LPS injection.
- AQP4 mRNA remained almost unchanged after LPS treatment.
- The expression of AQP4 protein was only slightly reduced at one day after LPS injection.
Takeaway
This study looked at how two proteins in the eye change when there is inflammation, which can cause swelling and problems with vision.
Methodology
The study used male Wistar rats, inducing uveitis with LPS injections and evaluating changes in protein and mRNA expression through various techniques including RT-PCR and Western blotting.
Participant Demographics
Male Wistar rats, aged 6-8 weeks.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
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