MIQuant: A Software for Assessing Infarct Size in Heart Injury Models
Author Information
Author(s): Diana S. Nascimento, Mariana Valente, Tiago Esteves, Maria de Fátima de Pina, Joana G. Guedes, Ana Freire, Pedro Quelhas, Perpétua Pinto-do-Ó
Primary Institution: Instituto de Engenharia Biomédica (INEB), Universidade do Porto, Porto, Portugal
Hypothesis
The study aims to standardize and simplify infarct size assessment using a novel semi-automated software tool called MIQuant.
Conclusion
MIQuant is a reliable and easy-to-use software for quantifying infarct size, which can help standardize assessments across studies.
Supporting Evidence
- MIQuant reduced the time spent on analysis by 4.5-fold compared to manual methods.
- High correlation (r=0.981 for midline length and r=0.970 for area) was found between MIQuant and manual measurements.
- Bland-Altman analysis showed acceptable limits of agreement between MIQuant and manual methods.
- MIQuant's effectiveness is independent of user proficiency.
Takeaway
MIQuant is a computer program that helps scientists measure heart damage more easily and quickly, making it better for comparing results from different studies.
Methodology
Mice underwent coronary artery ligation, and infarct size was measured using both manual planimetry and MIQuant software, with repeatability and reproducibility verified.
Potential Biases
Potential variability in infarct size measurements due to differences in user experience with the software.
Limitations
The study only used mice and one model of cardiac ischemia, which may limit the generalizability of the findings.
Participant Demographics
Male and female C57BL/6 mice aged 8 to 12 weeks were used.
Statistical Information
P-Value
p<0.01
Confidence Interval
1.21% to 3.72%
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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