Real-Time Imaging of Reactive Oxygen Species in Heart Cells
Author Information
Author(s): Li Yunbo, Shen Haiou, Zhu Hong, Trush Michael A., Jiang Ming, Wang Ge
Primary Institution: Virginia Tech
Hypothesis
Can chemiluminescence imaging detect reactive oxygen species (ROS) formation in cardiomyocytes?
Conclusion
The study demonstrates that cultured cardiomyocytes release ROS under normal conditions and that exposure to benzo(a)pyrene-1,6-quinone significantly increases ROS production.
Supporting Evidence
- ROS were detected in cultured H9c2 cardiomyocytes using a chemiluminescence imaging system.
- Adding benzo(a)pyrene-1,6-quinone increased ROS formation by 4-5 times.
- Cell viability was over 99% during the experiments.
- Superoxide dismutase and catalase completely abolished ROS responses.
- D3T treatment significantly inhibited both constitutive and BPQ-stimulated ROS formation.
Takeaway
Scientists used a special camera to see how heart cells make harmful substances called ROS, which can hurt the heart. They found that a chemical can make these substances increase a lot.
Methodology
The study used chemiluminescence imaging to detect ROS in cultured rat H9c2 cardiomyocytes after treatment with various chemicals.
Limitations
The study primarily used in vitro models, which may not fully represent in vivo conditions.
Participant Demographics
Rat H9c2 cardiomyocytes were used in the study.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website