CPT1C 5'UTR and Its Role in Energy Regulation
Author Information
Author(s): Ines Lohse, Patrick Reilly, Kathrin Zaugg
Primary Institution: Department of Radiation Oncology, University Hospital Zurich, Zurich, Switzerland
Hypothesis
The presence of an upstream open reading frame (uORF) in the 5' UTR of CPT1C mRNA regulates its translation in response to cellular energy availability.
Conclusion
CPT1C translation is repressed by an upstream open reading frame, and this repression can be relieved by glucose deprivation and palmitate treatment.
Supporting Evidence
- CPT1C mRNA contains an upstream open reading frame that inhibits translation.
- Translation repression is relieved under glucose deprivation and palmitate treatment.
- AMPK activity influences the regulation of CPT1C translation.
Takeaway
CPT1C has a special part in its code that can turn off its production when energy is low, but certain conditions can turn it back on.
Methodology
The study used luciferase reporter constructs to analyze the effects of the uORF on translation under different energy conditions.
Limitations
The study does not explore the long-term effects of uORF regulation on CPT1C function in vivo.
Digital Object Identifier (DOI)
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