Phospho-ERK and AKT status, but not KRAS mutation status, are associated with outcomes in rectal cancer treated with chemoradiotherapy
2011

Impact of KRAS and BRAF Mutations on Rectal Cancer Treatment Outcomes

Sample size: 70 publication 10 minutes Evidence: moderate

Author Information

Author(s): Janine M Davies, Dimitri Trembath, Allison M Deal, William K Funkhouser, Benjamin F Calvo, Timothy Finnegan, Karen E Weck, Joel E Tepper, Bert H O'Neil

Primary Institution: University of North Carolina at Chapel Hill

Hypothesis

KRAS and BRAF mutations would confer radioresistance and result in lesser response to RT and worse survival.

Conclusion

KRAS mutation was not associated with lesser response to chemoradiotherapy or worse overall survival, while high p-ERK expression was linked to better outcomes.

Supporting Evidence

  • 64% of patients had KRAS wild-type status.
  • High p-ERK levels were associated with improved overall survival.
  • 36% of patients experienced recurrence, mostly distant recurrences.
  • Median overall survival was 4.5 years.

Takeaway

This study looked at how certain gene mutations affect the treatment of rectal cancer. It found that having a KRAS mutation doesn't make treatment less effective, and higher levels of a protein called p-ERK can actually help patients do better.

Methodology

The study evaluated pre-radiotherapy tumor biopsies for KRAS and BRAF mutations and p-AKT and p-ERK expression using pyrosequencing and immunohistochemistry.

Limitations

The study had a small number of patients with BRAF mutations, limiting conclusions about its impact.

Participant Demographics

{"mean_age":58,"gender_distribution":{"male":42,"female":28},"race_distribution":{"white":52,"black":15,"other_or_unknown":3},"clinical_stage_distribution":{"stage_II":25,"stage_III":39,"stage_IV":6}}

Statistical Information

P-Value

0.6

Confidence Interval

95% CI 3.0-12.5

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1748-717X-6-114

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