ATM Localization and Gene Expression in the Adult Mouse Eye
Author Information
Author(s): Julia Leemput, Christel Masson, Karine Bigot, Abdelmounaim Errachid, Anouk Dansault, Alexandra Provost, Stéphanie Gadin, Said Aoufouchi, Marc Abitbol
Primary Institution: Université Paris-Descartes
Hypothesis
How does ATM prevent oxidative stress and participate in the maintenance of genomic integrity and cell viability of the adult retina?
Conclusion
ATM and its phosphorylated form are involved in protecting ocular cells from oxidative damage and maintaining their structure and function.
Supporting Evidence
- ATM protein was detected in the nuclei of all adult mice retinal cells.
- High levels of the activated phosphorylated form of ATM were detected in the cytoplasm of photoreceptor cells.
- ATM and its phosphorylated form are essential for the maintenance of ocular cell structure and function.
Takeaway
This study looks at a protein called ATM in the eyes of adult mice, which helps protect cells from damage caused by stress and keeps them healthy.
Methodology
The study used RT-PCR, in situ hybridization, western blot analysis, and immunohistochemistry to analyze ATM expression and localization in mouse ocular tissues.
Limitations
The study primarily focuses on mouse models, which may not fully represent human conditions.
Participant Demographics
Adult C57BL/6J mice were used in the study.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
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