Monoclonal Antibodies for EBNA1 and Their Use in Protein Purification
Author Information
Author(s): Sarah J. Duellman, Richard R. Burgess
Primary Institution: McArdle Laboratory for Cancer Research, University of Wisconsin-Madison
Hypothesis
Can monoclonal antibodies (mAbs) against EBNA1 be effectively used for immunoaffinity chromatography?
Conclusion
The study successfully developed a panel of high-affinity monoclonal antibodies against EBNA1 that can be used for gentle purification of EBNA1 and its associated proteins.
Supporting Evidence
- Eleven high affinity monoclonal antibodies were generated against EBNA1.
- Four of the antibodies were identified as polyol-responsive, allowing for gentle elution of proteins.
- The antibodies showed varying affinities for different regions of the EBNA1 protein.
Takeaway
Scientists created special antibodies that can grab onto a virus protein called EBNA1, helping to study it better without breaking it apart.
Methodology
Mice were immunized with EBNA1, and hybridoma cell lines were created to produce monoclonal antibodies, which were then tested for their ability to bind and purify EBNA1.
Limitations
The study does not explore the full range of potential interactions of EBNA1 with other proteins.
Participant Demographics
Mice used for antibody production were Balb/c ByJ strain.
Digital Object Identifier (DOI)
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