Using Light to Track Colorectal Cancer in Mice
Author Information
Author(s): Zabala Maider, Alzuguren Pilar, Benavides Carolina, Crettaz Julien, Gonzalez-Aseguinolaza Gloria, Ortiz de Solorzano Carlos, Gonzalez-Aparicio Manuela, Kramer Maria Gabriela, Prieto Jesus, Hernandez-Alcoceba Ruben
Primary Institution: University of Navarra
Hypothesis
Can bioluminescent imaging (BLI) effectively monitor colorectal cancer progression and response to immunogene therapy in a murine model?
Conclusion
Bioluminescent imaging is a rapid and safe method for monitoring tumor progression in the liver and evaluating experimental treatments like immunotherapy.
Supporting Evidence
- BLI was found to be more sensitive than ultrasonography for detecting small tumors.
- The study demonstrated that BLI can monitor tumor progression and response to treatment in real-time.
- BLI allowed for the identification of different responses to immunotherapy among treated mice.
Takeaway
Scientists used a special light technique to see how cancer grows in mice and how well new treatments work. This helps them understand cancer better without hurting the mice.
Methodology
Mice were injected with a colon cancer cell line that emits light, and tumor progression was monitored using bioluminescent imaging and compared with ultrasonography.
Limitations
BLI may be less reliable for assessing tumor burden in advanced stages of the disease due to a plateau in luciferase activity.
Participant Demographics
C57BL/6 mice were used in the study.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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