Isolation and Characterization of the First Microsatellite Markers for the Invasive Mussel Sinanodonta woodiana
2011

First Microsatellite Markers for the Invasive Mussel Sinanodonta woodiana

Sample size: 20 publication Evidence: moderate

Author Information

Author(s): Popa Oana Paula, Popa Luis Ovidiu, Krapal Ana-Maria, Murariu Dumitru, Iorgu Elena Iulia, Costache Marieta

Primary Institution: Molecular Biology Department, “Grigore Antipa” National Museum of Natural History

Hypothesis

The study aims to isolate and characterize microsatellite markers for Sinanodonta woodiana to aid in understanding its population genetics and invasion process.

Conclusion

The study successfully identified eight polymorphic microsatellite loci that can be used to investigate the population genetics of the invasive mussel Sinanodonta woodiana.

Supporting Evidence

  • Eight polymorphic microsatellite loci were successfully isolated for Sinanodonta woodiana.
  • The number of alleles per locus ranged from 7 to 14.
  • Observed heterozygosity ranged from 0.650 to 0.950.
  • All loci exhibited Hardy-Weinberg equilibrium.

Takeaway

Scientists found special markers in a type of mussel that help them understand how it spreads and survives in new places.

Methodology

Microsatellite loci were developed using a modified enrichment protocol, and genetic screening was performed on 20 individuals collected from the Prut River.

Limitations

The study is limited to a specific population in Romania and may not represent genetic diversity in other regions.

Participant Demographics

Individuals were collected from the Prut River in Romania.

Digital Object Identifier (DOI)

10.3390/ijms12085255

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